RPB
Guide 01

Reconstitution, step by step

Turning a lyophilised (freeze-dried) powder into a solution you can measure accurately. Ten minutes, done once per vial.

Reviewed 3 Oct 2026 by A.N.
8 min read · EN ES DE FR PT ZH
CC BY-NC-SA 4.0
Before you start
  • The vial and its label (amount in mg)
  • — or sterile water if the profile says so
  • Alcohol swabs and a clean, flat surface
  • A insulin syringe to draw the
  • The calculator open, with your numbers entered
Read the compound’s profile first — storage and stability differ.

Steps

  1. Check the label

    Confirm the amount (mg) in the vial and the expiry. Write the lot number in your vial log.

    Where you got it isn’t recorded here, on purpose.

  2. Let it reach room temperature

    Take the vial from the fridge 10–15 minutes before. Cold glass and warm liquid can stress the peptide.

  3. Swab both tops

    Wipe the rubber stopper of the vial and of the with alcohol. Let them dry.

  4. Draw the diluent

    Draw exactly the volume you entered in the calculator. Tap out bubbles.

  5. Add it slowly, down the glass

    Angle the needle against the inside wall so the liquid runs down the side, not onto the powder.

    Squirting directly onto the powder is the most common way to damage it.

  6. Swirl, don’t shake

    Roll the vial gently between your fingers until clear. Some compounds take a few minutes.

  7. Check the solution

    It should be clear (GHK-Cu is clear blue). Cloudiness or particles mean something is wrong.

  8. Label and date it

    Write the date and concentration on the vial. Your log calculates the discard date.

  9. Refrigerate

    Store as the profile says, protected from light.

Worked example

Vial
5 mg
Diluent
2 ml
Concentration
2.5 mg/ml
250 mcg =
10 units

250 mcg is 0.25 mg. 0.25 ÷ 2.5 = 0.1 ml. On a syringe, 0.1 ml is 10 units. Run your own numbers →

Common mistakes

Unit confusion. mg vs mcg is a factor of 1,000. Always say the unit out loud when you check.
Wrong diluent volume. If you add 3 ml but calculated for 2 ml, every dose after that is off by a third.
Shaking the vial. Foam and agitation can break peptide bonds.
Re-using needles. Blunt, non-sterile, and a route for contamination into the vial.
No date on the vial. You won’t remember in three weeks.